Fig. 8

SOD isoform expression during extended DPSC culture with or without exogenous H₂O₂ (50–200 μM) treatment. a–c qRT-PCR analysis of SOD1, SOD3, and SOD2 gene expression by high proliferative (10–25PDs and 40–65PDs) and low proliferative (2–10PDs) DPSC sub-populations. Relative fold changes in enzymic antioxidant gene expression (RQ) were calculated using the 2^–ΔΔCt method, normalized versus an 18S rRNA housekeeping gene. N = 3, values in the graphs represent mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001