Fig. 3From: Regulation of connective tissue growth factor expression by miR-133b for the treatment of renal interstitial fibrosis in aged mice with unilateral ureteral obstructionCTGF is a direct target gene of miR-133b. Transient transfection of miR-133b mimic into HK2 cells for 48 h. A miR-133b expression was detected by real-time PCR. B CTGF expression was detected by real-time PCR. C CTGF expression was analyzed by western blotting. D Dual-luciferase reporter assay confirmed CTGF as a direct target gene of miR-133bBack to article page