Fig. 2

hsa_circ_0006859 suppresses osteoblastic differentiation. a Relative hsa_circ_0006859 expression in hBMSCs during osteogenic differentiation. b Relative hsa_circ_0006859 expression in hBMSCs after transfection with OE-circ or si-circ. c Relative OCN mRNA expression in hBMSC after transfection with OE-circ or si-circ. d Relative ALP mRNA expression in hBMSCs after transfection with OE-circ or si-circ. e Western blot analysis of OCN and ALP expression in hBMSCs after transfection with OE-circ or si-circ. f ALP activity in hBMSCs after transfection with OE-circ or si-circ. g Alizarin red staining was used to detect the mineralization ability of hBMSCs after transfection with OE-circ or si-circ. ***P < 0.0001