Fig. 4

hsa_circ_0006859 directly binds to miR-431-5p. a Bioinformatics analysis of the predicted binding sites between hsa_circ_0006859 and miR-431-5p. b Luciferase activity of hsa_circ_0006859 circ-#1-Wt and circ-#1-Mut upon miR-431-5p overexpression. c Luciferase activity of hsa_circ_0006859 circ-#2-Wt and circ-#2-Mut upon miR-431-5p overexpression. d Relative miR-431-5p expression in hBMSC after transfection with OE-circ or si-circ. e RNA pull-down assay of the interaction between hsa_circ_0006859 and miR-431-5p