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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Exosomal hsa_circ_0006859 is a potential biomarker for postmenopausal osteoporosis and enhances adipogenic versus osteogenic differentiation in human bone marrow mesenchymal stem cells by sponging miR-431-5p

Fig. 5

hsa_circ_0006859 acts as a competing endogenous RNA (ceRNA) to promote ROCK1 expression. a Bioinformatics analysis of the predicted binding sites between miR-431-5p and ROCK1. b Luciferase activity of ROCK1-Wt and ROCK1-Mut upon miR-431-5p overexpression. c Relative ROCK1 mRNA expression in hBMSCs after transfection with miR-431 M or miR-431I. d Western blot analysis of ROCK1 expression in hBMSCs after transfection with miR-431 M or miR-431I. e Luciferase activity of ROCK1-Wt and ROCK1-Mut in hBMSCs after transfection with miR-NC, si-circ or si-circ+miR-431I. f Relative ROCK1 mRNA expression in hBMSCs after transfection with miR-NC, si-circ, or si-circ+miR-431I

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Stem Cell Research & Therapy

ISSN: 1757-6512