Fig. 6

hsa_circ_0006859 suppresses osteogenesis and promotes adipogenesis by sponging miR-431-5p to upregulate ROCK1. a Relative miR-431-5p expression in hBMSCs after transfection with miR-431 M or miR-431I. b ALP activity in hBMSCs after transfection with miR-431 M or miR-431I. c Alizarin red staining was used to detect the mineralization ability of hBMSCs after transfection with miR-431 M or miR-431I. d Oil Red O staining of hBMSCs after transfection with miR-431 M or miR-431I. e Relative ROCK1 mRNA expression in hBMSCs after transfection with OE-ROCK1, si-ROCK1 #1, si-ROCK1 #2, or si-ROCK1 #3. f Western blot analysis of ROCK1 expression in hBMSCs after transfection with OE-ROCK1, si-ROCK1 #1, si-ROCK1 #2, or si-ROCK1 #3. g Alizarin red staining of hBMSCs after transfection with OE-ROCK1 or si-ROCK1. h Oil Red O staining of hBMSCs after transfection with OE-ROCK1 or si-ROCK1. i Alizarin red staining of hBMSCs after transfection with si-NC+EV, si-NC+OE-circ, si-ROCK1+EV, or si-ROCK1+OE-circ. j Oil Red O staining of hBMSCs after transfection with si-NC+EV, si-NC+OE-circ, si-ROCK1+EV, or si-ROCK1+OE-circ