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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Inhibition of miR-199a-5p rejuvenates aged mesenchymal stem cells derived from patients with idiopathic pulmonary fibrosis and improves their therapeutic efficacy in experimental pulmonary fibrosis

Fig. 4

miR-199a-5p induced IPF-MSC senescence by regulating autophagy. a Representative images of autophagosomes examined using a TEM and quantitative analysis of autophagosomes in control-MSCs transfected with miR control, miR-199a-5p mimic, or miR-199a-5p mimic + rapamycin. Scale bar = 1 μm. b Western blotting analysis of p62, Beclin, LC3II/I, p21, and p16 protein expression in control-MSCs transfected with miR control, miR-199a-5p mimic, or miR-199a-5p mimic + rapamycin. c Representative images and quantitative analysis of SA-β-gal staining in control-MSCs transfected with miR control, miR-199a-5p mimic, or miR-199a-5p mimic + rapamycin. Scale bar = 200 μm. d Representative images of autophagosomes examined using a TEM and quantitative analysis of autophagosomes in IPF-MSCs transfected with miR control, miR-199a-5p inhibitor, or miR-199a-5p inhibitor +3MA. Scale bar = 1 μm. e Western blotting analysis of p62, Beclin, LC3II/I, p21, and p16 protein expression in IPF-MSCs transfected with miR control, miR-199a-5p inhibitor, or miR-199a-5p inhibitor +3MA. f Representative images and quantitative analysis of SA-β-gal staining in IPF-MSCs transfected with miR control, miR-199a-5p inhibitor, or miR-199a-5p inhibitor +3MA. Scale bar = 200 μm. All data were obtained from at least three independent experiments and each error bar represents the mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001

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