Fig. 1
From: hiPSC-derived NSCs effectively promote the functional recovery of acute spinal cord injury in mice
The characteristic of hiPSC-NSC and huMSC. a The hiPSC-NSC were positive for nestin, SOX2 and PAX6. b Cell surface antigens were detected at passage 4 using flow cytometry. huMSCs were positive for CD29, CD44, CD73, CD90, and CD105. In contrast, very little expression of the haematopoietic lineage markers HLA-DR and CD45 was detected in huMSCs. c Oil red O staining of lipid droplets revealed that huMSCs differentiated into cells of the adipogenic lineage. Bar, 50 μm. d Alizarin red staining revealed a calcified extracellular matrix of induced huMSCs, confirming that huMSCs differentiated into osteogenic cells. Bar, 500 μm. e Safranin O staining showed the secretion of the extracellular matrix of chondrocytes