Skip to main content
Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: MSCs derived from amniotic fluid and umbilical cord require different administration schemes and exert different curative effects on different tissues in rats with CLP-induced sepsis

Fig. 4

The administration of MSCs improved CLP-induced histological and functional changes in the liver of septic rats, while UC-MSCs performed better in reducing inflammatory cell infiltration than AF-MSCs. The histological changes in the liver were identified by performing HE (a), Masson’s trichrome (b) and PAS (c) staining. In a, a representative image of the liver CV is shown in the upper panel, and the capsule is shown in the lower panel. Necrotic and degenerative hepatocytes were observed near the CV, and inflammatory cell infiltration into the capsule (black arrow) was evident in the sepsis group. The administration of MSCs improved CLP-induced cell death (as determined by the morphology), capsule inflammation, swelling of vascular endothelial cells and reduction in glycogen levels within hepatocytes, while inflammatory cell infiltration was still present in the AF-MSC group. No difference in cell death in the liver was detected among the four groups, as assessed using TUNEL staining (d and e). Other assessments of liver function included measurements of the AST/ALT ratio (f). Both types of MSCs relieved the CLP-induced increase in this parameter. CV: central vein; n: necrotic hepatocytes with an eosinophilic cytoplasm and pyknotic nucleus; d1, degenerating cells with a loss of the intact cell morphology; d2, degenerative area with understained cytoplasm. For images shown in the upper panels of a, b and c, scale bar = 20 μm; for images shown in the lower panels of a, scale bar = 50 μm; for d, scale bar = 100 μm. *p < 0.05; ns, no significant difference

Back to article page