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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: GPR4 signaling is essential for the promotion of acid-mediated angiogenic capacity of endothelial progenitor cells by activating STAT3/VEGFA pathway in patients with coronary artery disease

Fig. 3

Expression of the proton-sensing GPCR family and angiogenic capacity in EPCs derived from non-CAD and CAD individuals. a dPCR was used to determine the mRNA level of proton-sensing GPCR family expression in EPCs (n = 6). b, c Representative photographs and quantitative analyses of the protein levels of the GPCR family (n-6). d dPCR was used to determine the mRNA level of GPR4 in different pH environments (n = 6). e Quantitative analyses and representative photographs of the expression of GPR4 in different pH environments, followed by immunoblotting with a GPR4 antibody. f, g EPCs were transfected with siRNA-NC or siRNA-GPR4, representative photographs and quantitative analyses of the Matrigel tube-like formation test (scale bar = 40 μm, n = 6) and scratch test (scale bar = 20 μm, n = 6). The data are presented as the mean ± SD. **P < 0.01, ***P < 0.001

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Stem Cell Research & Therapy

ISSN: 1757-6512