Skip to main content
Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: METTL3-mediated m6A modification regulates cell cycle progression of dental pulp stem cells

Fig. 6

METTL3 regulated cell cycle via PLK1 m6A modulation. a Predicted protein-protein interaction (PPI) network of overlapping genes was assayed in the STRING database and displayed by Cytospcape which identified PLK1 as one of the key nodes in PPIs. b mRNA expression of PLK1 in DPSCs after METTL3 knockdown and overexpression detected by PCR. c Protein expression of PLK1 assayed by Western blot. d Visual data of highly enriched m6A peaks in PLK1 detected by m6A RIP-seq. e m6A RIP-qPCR demonstrated m6A-methylated level of PLK1 sharply decreased after METTL3 inhibition. f Flow cytometry analysis evaluated cell cycle portions in shMETTL3-DPSCs treated with 1 nM PLK1 inhibitor BI2536 or DMSO as negative control. Data are represented as mean ± SD (n = 3), Student’s t test. *p < 0.05. **p < 0.01. ***p < 0.001

Back to article page