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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Effect of fibronectin, FGF-2, and BMP4 in the stemness maintenance of BMSCs and the metabolic and proteomic cues involved

Fig. 2

The expression and translocation pattern of OCT4, SOX2, and c-MYC with the stimulation of FN + FGF2 + BMP4. a–c All treatments transiently induced the expressions of OCT4, SOX2, and c-MYC, and the induction effect of FN + FGF2 + BMP4 was most obvious. d, e Treated cells with FN + FGF2 + BMP4 enhanced the protein expression of OCT4, SOX2, and c-MYC. The primary antibody of each target protein OCT4 (f–i), SOX2 (j–m), and c-MYC c (n–q) was detected with Alexa Fluor 488 (green stain). Nuclear translocation of the target transcription factors was observed in all treated samples (FN + FGF2 + BMP4) at passage 5. Statistical significance was accepted at *p < 0.05 or **p < 0.001

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Stem Cell Research & Therapy

ISSN: 1757-6512