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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Osthole enhances the bone mass of senile osteoporosis and stimulates the expression of osteoprotegerin by activating β-catenin signaling

Fig. 3

Osthole inhibits osteoclastogenesis in a dose-dependent and OPG-dependent manner. a Osteoblasts were cultured and treated with various doses of osthole (10–100 μM) or vehicle (DMSO) for 2 days; OPG expression was detected using real-time PCR assay. b The protein expression of OPG was detected using Western blot assay. a, b showed that osthole significantly increased the mRNA and protein level of OPG in a dose-dependent manner. BMSCs were isolated from 3-month-old OPG−/− mice and the littermates of OPG+/+ mice, TRAP staining was performed. The scar bar is 100 μm. Quantification of osteoclast number for osthole in c. The number of multinucleated TRAP-positive cells (> 3 nuclei) in each representative view area at × 40 magnification. *P < 0.05, unpaired Student's t test. c, d Osthole inhibited the formation of osteoclasts in an OPG-dependent manner

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