Fig. 1
From: The CAD risk locus 9p21 increases the risk of vascular calcification in an iPSC-derived VSMC model
Characterization of WT iPSCs and derived VSMCs. a NR (top) and R (bottom) WT iPSCs show similar iPSC morphology, as seen in the phase contrast (PhC) images. Scale bars represent 100 μm. Staining with the pluripotency marker NANOG (green) confirmed nuclear expression, as seen in the overlaid image (merge). Nuclei were counterstained with DAPI (blue). Scale bars represent 50 μm. b NR (top) and R (bottom) WT iPSC-derived VSMCs show colocalization of CNN1 (red) and TAGLN (green) in the contractile apparatus of the cells. No differences were observed between R and NR WT VSMCs. Nuclei were counterstained with DAPI. Scale bars represent 50 μm