Fig. 5
From: The CAD risk locus 9p21 increases the risk of vascular calcification in an iPSC-derived VSMC model
Calcification-associated markers are increased in R WT, but decreased in NR WT calcifying VSMCs. a–e R WT-derived calcifying VSMCs show increased expression of calcification markers ALPL (a), CSF1 (b), and CTSK (c) (clone 1 (orange)) and decreased or unchanged expression in the other two clones (blue and green). RUNX2 (d) was upregulated significantly in two out of three R WT clones. OPN (e) expression did not change significantly. f–l In NR WT-derived calcifying VSMCs, the markers ALPL (f), CSF1 (g), and CTSK (h), and RUNX2 (k) were downregulated in one or both clones. OPN (l) expression was unchanged in NR WT cells