Table 1 Antibodies used for IF
From: The CAD risk locus 9p21 increases the risk of vascular calcification in an iPSC-derived VSMC model
Antibody | Host species | Dilution | Manufacturer | Cat. number |
|---|---|---|---|---|
hCNN1 | Mouse | 1:500 | Sigma | C2687 |
hKi67 (marker of proliferation Ki67) | Mouse | 1:300 | BD Pharmingen | 556003 |
hNANOG | Rabbit | 1:300 | Cell Signaling | 4903 |
hTAGLN | Rabbit | 1:500 | Abcam | ab14106 |
Anti-rabbit IgG | Donkey | 1:300 | Invitrogen | A21206 |
Anti-mouse IgG | Goat | 1:500 | Invitrogen | A11029 |
Anti-mouse IgG | Donkey | 1:500 | Invitrogen | A10037 |