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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Anti-apoptotic effect of adipose tissue-derived stromal vascular fraction in denervated rat muscle

Fig. 3

Anti-apoptotic effects of the SVF cell secretome on TNF-α-induced apoptosis. a Western blot showing the effects of SVF on the activation of caspase-3 and caspase-7 as well as the cleavage of PARP in myoblasts treated with 10 ng/ml TNF-α for 24 h. b, c Caspase 3/7 activity (b) and caspase 8 activity (c) in TNF-α treated myoblasts (10 ng/ml) after 24 h of indirect co-culture with SVF. d The percentage of Annexin-V positive myoblasts in SVF co-cultures after 24 h of TNF-α treatment (10 ng/ml). e The percentage of TUNEL-positive myoblasts in SVF co-cultures after 48 h of TNF-α treatment (10 ng/ml). n = 6. f TUNEL labeling in myoblasts indirectly co-cultured with SVF cells. n = 3. DAPI staining shown in the upper left corner. × 20 magnification. Statistical significance was calculated using one-way ANOVA with post hoc test (Bonferroni correction). **P < 0.01, ***P < 0.001. ns = not significant

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