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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Anti-apoptotic effect of adipose tissue-derived stromal vascular fraction in denervated rat muscle

Fig. 4

TNF-α-induced apoptosis is mitochondria-independent in vitro. a Cytochrome c levels in the mitochondria (left) and the cytosol (right) of myoblasts at different time points (0–6 h) after TNF-α treatment (10 ng/ml). TFAM was used as a housekeeping gene for the mitochondria. b The expression of mitochondria-related proteins Bax (pro-apoptotic) and Bcl-2 (anti-apoptotic) after 4 h of TNF-α treatment. c The expression of Bax and Bcl-2 protein after 24 h of TNF-α treatment

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