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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Extracellular vesicles derived from EphB2-overexpressing bone marrow mesenchymal stem cells ameliorate DSS-induced colitis by modulating immune balance

Fig. 1

Characterization of rat EphB2-transfected BMSCs and EVs. a Representative image of Passage 3 (P3) BMSCs. Original magnification, × 100. b Flow cytometry characterization of P3 BMSCs as CD45 CD11b CD90+ CD29+. c Transmission electron microscopy (TEM) analysis of EVs derived from BMSCs. Scale bar, 200 nm. d The size distribution profile of the EVs by NanoSight. e Western blotting analysis of EVs specific marker proteins CD9, TSG101, and CD63 in BMSCs and BMSCs-derived EVs. f Fluorescence microscope imaging of null-BMSCs and GFP-labeled EphB2-BMSCs. Original magnification, × 100. g qRT-PCR analysis of EphB2 expression in the indicated cells (left) or EVs (right). The expression level of the mRNA was normalized to β-actin. **P < 0.01 vs. DSS group

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