Fig. 2

EphB2/ephrin-B1 axis promotes the homing of EVs to inflammatory colon tissue. a Confocal microscopy analysis of the localization of PKH26-labeled EVs and EphB2-EVs (red) in inflamed colon tissues. The cell nuclei were stained with DAPI (blue). Scale bar, 100 μm. Magnification images scale bar, 50 μm. b The number of PKH26-labeled EVs in each microscopic field was counted (magnification × 400), and 5 horizons were selected for each sample. qRT-PCR analysis of EphB2 (c) and ephrin-B1 (d) expression in colon tissues with indicated treatments. The expression level of the mRNA was normalized to β-actin. e Western blotting analysis of EphB2 and ephrin-B1 expressions in colon tissue. β-Actin was used as a loading control. ***P < 0.001 vs. null-EVs group, ^##P < 0.01 vs. normal group, **P < 0.01 vs. DSS group, ^△△P < 0.01 vs. EVs group