Fig. 1
From: Robust expansion and functional maturation of human hepatoblasts by chemical strategy
Establishment of a chemical culture cocktail for HB expansion. a Schematic of the strategy to identify chemical culture cocktail for HB expansion. b Immunostaining analyses of HB-specific marker AFP and HNF4α expression after different chemical cocktails treated. Scale bars 100 μm. c Efficiency of AFP and HNF4α expression after different chemical cocktails treatment, determined by counting positive cells. Efficiencies are presented as the percentage of positive cells plus or minus the SD of all fields counted. d Quantitative RT-PCR analyzed hepatic genes and Ki67 expression in chemical cocktails treated HBs. Data are presented as mean ± SEM, n = 3. *P < 0.05, **P < 0.01. e Immunostaining analyses of AFP and HNF4α expression in chemical cocktails treated HBs. Scale bars 100 μm. f Efficiency of AFP and HNF4α expression after different chemical cocktails treatment, determined by counting positive cells. Efficiencies are presented as the percentage of positive cells plus or minus the SD of all fields counted