Fig. 2
From: Robust expansion and functional maturation of human hepatoblasts by chemical strategy
The ACDFSV culture condition enables long-term self-renewal and maintenance of HBs. a Phase-contrast images of expanding HBs. Scale bar 100 μm. b Growth curve of HBs cultured in ABCEHS cocktail and ACDFSV cocktail conditions. Cell growth curves were analyzed by obtaining a cell count. Data are presented as mean ± SEM, n = 3. c Immunostaining analyses of AFP and Ki67 expression on expanded HBs (passage 10). Scale bars 50 μm. d Flow cytometric analyses of AFP and Ki67 expression in expanded HBs (passage 10). e Immunostaining analyses of HB marker proteins on expanded HBs (passage 10). Scale bars 100 μm. f Expanded HBs differentiated into ALB and E-CAD co-staining hepatocytes after mature induction. Scale bars 50 μm. g Expanded HBs differentiated into CK19 and F-actin co-staining bile duct-like structures in 3D culture. Scale bar 20 μm