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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Robust expansion and functional maturation of human hepatoblasts by chemical strategy

Fig. 3

Establishment of a chemical induction cocktail for HLC maturation. a Schematic of screening induction condition for hepatic maturation. b The morphology of expanded HB-derived HLCs. HLCs exhibited distinct round nuclei even some has two nuclei (white arrows). Scale bar 100 μm. c Quantitative RT-PCR analyzed AFP, ALB, and A1AT expression in HLCs derived from different induction cocktails. Gene expression was normalized to HBs. Data are presented as mean ± SEM, n = 3. *P < 0.05, **P < 0.01. d Immunostaining analyses of hepatocyte markers ALB, E-CAD, and A1AT on HLCs derived from different induction cocktails. Scale bars 100 μm. e Flow cytometric analyses for ALB and E-CAD positive rate on HLCs derived from different induction cocktails

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