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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: H3K9ac of TGFβRI in human umbilical cord: a potential biomarker for evaluating cartilage differentiation and susceptibility to osteoarthritis via a two-step strategy

Fig. 5

Decreased expression and H3K9ac levels of TGFβRI were verified in the rats with low birth weight. a Immunofluorescence analysis of TGFβRI in the cartilage of male IUGR rat offspring caused by prenatal caffeine, nicotine, ethanol, and dexamethasone exposure, scale bar = 50 μm. b RT-qPCR analysis of TGFβRI in the cartilage of male offspring with IUGR caused by prenatal caffeine, nicotine, ethanol, and dexamethasone exposure. n = 6. c ChIP-PCR analysis of TGFβRI in the cartilage of male offspring with IUGR caused by prenatal caffeine, nicotine, ethanol, and dexamethasone exposure. n = 3. GD, gestational day; PW6, postnatal week 6; DAPI, 4′,6-diamidino-2-phenylindole; RT-qPCR, real-time quantitative polymerase chain reaction; TGFβRI, transforming growth factor β receptor I; ChIP-PCR, chromatin immunoprecipitation-polymerase chain reaction. Data are the mean ± S.E.M. #P < 0.05, ##P < 0.01 vs before the second hit, namely intra-articular papain injection, strenuous running, or ovariectomy; *P < 0.05, **P < 0.01 vs control

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