H3K9ac of TGFβRI in human umbilical cord: a potential biomarker for evaluating cartilage differentiation and susceptibility to osteoarthritis via a two-step strategy

Table 1 Oligonucleotide primers used for RT-qPCR conditions

Genes	Forward primer	Reverse primer	Annealing
Homo GAPDH	GAAATCCCATCACCATCTTCCAG	GAGTCCTTCCACGATACCAAAG	60
Homo COL2A1	GCTCCCAGAACATCACCTACCA	ACAGTCTTGCCCCACTTACCG	60
Homo ACAN	AAGGGCGAGTGGAATGATGT	CGCTTCTGTAGTCTGCGTTTGT	60
Homo TGFβRI	GCAATGGGCTTAGTATTCTGGG	TCCTGTTGACTGAGTTGCGATAAT	60
Homo Smad2	TCTGGGCAGCCGTAAGTTTA	CCACTGTTGCGACGATTAGG	60
Homo Smad3	CGGTTCACAAGGCTCAAGAG	AAGTGGGTCCTCAGAAGTGG	60
Homo MMP3	AATCAATTCTGGGCTATCAGAGG	GCATCAATCTTTGAGTCAATCCC	60
Homo MMP13	CAGAACTTCCCAACCGTATTGAT	TGTATTCAAACTGTATGGGTCCG	60
Homo ADAMTS5	TTTCTCCAAAGGTGACCGATG	CCTCCACATACTCCGCACTTG	60
Rat GAPDH	GCAAGTTCAACGGCACAG	GCCAGTAGACTCCACGACA	60
Rat TGFβRI	CTCGAGCAGTTACAAAGGGC	CTCGAGCAGTTACAAAGGGC	60

GAPDH, glyceraldehyde phosphate dehydrogenase; COL2A1, α1 chain of type II collagen; ACAN, Aggrecan; TGFβRI, transforming growth factor β receptor I; MMP3, matrix metalloproteinase 3; MMP13, matrix metalloproteinase 13; ADAMTS5, a disintegrin and metalloprotease with thromospondinmotifs 5

ISSN: 1757-6512