Skip to main content
Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: GSK3β rephosphorylation rescues ALPL deficiency-induced impairment of odontoblastic differentiation of DPSCs

Fig. 6

LiCl rescued odontoblastic differentiation of HPP DPSCs were blocked by β-catenin siRNA. a, b The β-catenin silence efficiency was examined by RT-PCR (a) and Western blotting (b) in HPP DPSCs with β-catenin siRNA transfection for 48 h (n = 3). c The expression of t-GSK3β and p-GSK3β, and the total and active β-catenin were examined by Western blotting in HPP DPSCs from the NaCl control, LiCl+Nc siRNA, and LiCl+β-catenin siRNA groups, which were cultured in odontoblastic medium for 7 days. d, e To evaluate LiCl rescue odontoblastic differentiation of HPP DPSCs by canonical Wnt pathway, the cells were treated with LiCl meanwhile transected with β-catenin siRNA. The odontoblastic differentiation was examined by Alizarin Red staining (d) after in odontoblastic medium for 3 weeks, and Western blotting (e) after in odontoblastic medium for 7 days. The data were presented as the mean ± s.d. of triplicate samples and analyzed by Student’s t test or one-way ANOVA. **p < 0.01, ***p < 0.001

Back to article page