Fig. 7

GSK3β rephosphorylation ameliorated tooth associated defects in ALPL^+/− mice with LiCl treatment. a Immunofluorescence staining of p-GSK3β and active β-catenin was performed in the first molars of ALPL^+/− mice and ALPL^+/− mice with LiCl treatment. Scale bar = 100 μm. b The micro-CT analysis of the low jaws including the molars, alveolar bone height (arrowheads), bone mass of alveolar bone and mandible bone (arrows), and DMD was performed in ALPL^+/− mice and ALPL^+/− mice treated with LiCl. Scale bar = 2 mm. c The histology of dentoalveolar structures including acellular cementum (AC), periodontal ligament (PDL), dentin (DE), and alveolar bone (AB) was analyzed in the first molars of ALPL^+/− mice and ALPL^+/− mice treated with LiCl. Scale bar = 100 μm. d SEM analysis the ultra-structures of dentinal tubules in the first molars from ALPL^+/− mice and ALPL^+/− mice treated with LiCl, and the dentinal tubules with exposure of collagen network of ALPL^+/− mice were significantly improved after LiCl therapy as show by the arrows. n = 8 mice per group. The data were presented as the mean ± s.d. of triplicate samples and analyzed by Student’s t test. **p < 0.01