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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Improvement of ECM-based bioroot regeneration via N-acetylcysteine-induced antioxidative effects

Fig. 3

The effect of NAC on the proliferation, migration and osteogenic differentiation of hDFCs under H2O2-induced oxidative stress. a Effect of combination drug treatment on the viability of hDFCs. b After 24 h of culture in Transwell plates, crystal violet staining was performed. c Pretreatment with NAC led to increased hDFC migration to the opposite side of the membrane compared with that observed in the H2O2-treated groups. d The effect of NAC on the osteogenic differentiation of hDFCs stimulated with H2O2, as determined by Alizarin Red staining. e RT-PCR analysis of Collagen I, ALP, RunX2, and Periostin mRNA expression. P < 0.05, P < 0.01, and P < 0.001

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