Fig. 4
From: Generation of UCiPSC-derived neurospheres for cell therapy and its application
Neurospheres strengthen survival of AT-exposed iNSCs. a iNSC monolayer was cultured to form neurospheres and exposed to AT for D3 and D7. b The survival rates of monolayer-iNSCs/AT and neurospheres/AT were compared at different time points (n = 3). c The neurospheres/D7 were isolated and then differentiated into neurons. Neurons were stained using TUJ1. d The survival of neurospheres in 10, 20, and 30 °C, respectively. d Comparison of three methods of storing iNSCs (n = 3). ***indicates P  < 0.001, ns indicates no statistical significance. Scale bars: 100 μm (A: neurospheres; neuron/D1); 50 μm (A: neuron/D30)