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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Morusin induces osteogenic differentiation of bone marrow mesenchymal stem cells by canonical Wnt/β-catenin pathway and prevents bone loss in an ovariectomized rat model

Fig. 1

The impacts of Morusin on proliferation and osteogenic differentiation of BMSCs. a The chemical structure of Morusin. b Detection of the proliferation toxicity of Morusin on BMSCs via MTT assay. BMSCs were cultured in osteogenic growth medium (OGM) with diverse concentrations of Morusin for 3, 5, 7, and 14 days. c–f mRNA level of marker genes for osteogenesis. Quantitative real-time PCR analysis of gene expression of osteogenic differentiation markers. We incubated BMSCs with Morusin of diverse concentrations (0–50 μM) for 3 days to observe the impact of Morusin on osteogenic differentiation. BMSCs, bone marrow mesenchymal stem cells; RUNX2, runt-related transcription factor 2; COL1A1, collagen type I alpha 1; BSP, bone sialoprotein; OCN, osteocalcin. We repeated the independent experiment for three times, with similar results achieved each time. Means ± SEM. *P < 0.05 and **P < 0.01 in comparison to the control group

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Stem Cell Research & Therapy

ISSN: 1757-6512