Fig. 2From: Morusin induces osteogenic differentiation of bone marrow mesenchymal stem cells by canonical Wnt/β-catenin pathway and prevents bone loss in an ovariectomized rat modelInfluences of Morusin on osteogenic differentiation of BMSCs. a Detection of the expression of osteo-specific genes (Col1a1, Runx2, and Bsp) at days 3, 5, and 7 of osteogenic differentiation via quantitative real-time PCR. We normalized the gene expression levels to GAPDH. b Relative protein expression of osteo-specific genes (COL1A1 and RUNX2) at days 3, 5, and 7 of osteogenic differentiation. The protein expression level is the gray value ratio of the target protein to GAPDH. c ALP staining of the osteogenic BMSCs. Scale bar, 250 μm. d Alizarin red staining of the osteogenic BMSCs. Scale bar, 250 μm. BMSCs, bone marrow mesenchymal stem cells; RUNX2, runt-related transcription factor 2; COL1A1, collagen type I alpha 1; BSP, bone sialoprotein. We repeated the independent experiment for three times. Means ± SEM. *P < 0.05 and **P < 0.01 in comparison to the control group. “ns” refers to no statistical significance compared with the control groupBack to article page