Fig. 4

The effect of Morusin on phosphorylation and nuclear transport of β-catenin. a Immunofluorescence assay of β-catenin in the osteogenic BMSCs. Morusin activates nuclear transposition of β-catenin in BMSCs. Scale bars, 100 μm. b Total and phosphorylated β-catenin in the osteogenic BMSCs treated with Morusin (10 μM) by Western Blotting. The protein expression level is the gray value ratio of the target protein to GAPDH. c Total and phosphorylated β-catenin in the osteogenic BMSCs treated with Morusin (10 μM) and DKK-1 (500 ng /ml) by Western Blotting. The protein expression level is the gray value ratio of the target protein to GAPDH. BMSCs, bone marrow mesenchymal stem cells; NC, negative control; p-β-catenin, phosphorylated β-catenin. We repeated the independent experiment for three times. Means ± SEM. *P < 0.05 and **P < 0.01 in comparison to the control group. “ns” refers to no statistical significance compared with the control group