Morusin induces osteogenic differentiation of bone marrow mesenchymal stem cells by canonical Wnt/β-catenin pathway and prevents bone loss in an ovariectomized rat model

Table 1 The primer sequences of genes in this experiment

Target genes	Forward primer	Reverse primer
COL1A1	TGTCGTTCAACGGCACAG	TGTGGTAGACTCCACGACA
RUNX2	GAGCGTTCAACGGCACAG	GACAGTAGACTCCACGACA
BSP	CGTCTCCATGGTGGATTATG	GGATGTAGTTCTGCTCATGG
OCN	GTCAGACTACAACATCCAGAAG	CGAGTATCTTCCTGTTTGACC
β-catenin	GCCATCACCACGCTGCATAATCT	GGCAGTCTGTCGTAATAGCCAAGAA
Dvl-1	CCTTCCATCCAAATGTTGCCAGTA	GGGCAGCCTCATCACGGTTT
LEF-1	CACACAACTGGCATCCCTCATC	GCTCCTGTTCCTTTCTCTCTGTTCGT
GAPDH	GGGTGTGAACCACGAGAAAT	ACTGTGGTCATGAGCCCTTC

COL1A1 collagen type I alpha 1, RUNX2 runt-related transcription factor 2, BSP bone sialoprotein, OCN osteocalcin, Dvl-1 dishevelled-1, LEF-1 lymphoid enhancing factor-1, GAPDH glyceraldehyde phosphate dehydrogenase

ISSN: 1757-6512