Characterization of stable hypoxia-preconditioned dental pulp stem cells compared with mobilized dental pulp stem cells for application for pulp regenerative therapy

Zayed, Mohammed; Iohara, Koichiro; Watanabe, Hideto; Ishikawa, Mami; Tominaga, Michiyo; Nakashima, Misako

doi:10.1186/s13287-021-02240-w

Stem Cell Research & Therapy

Table 2 Safety evaluation by blood tests and blood chemistry examinations at 1, 2 and 4 weeks and urinalysis at 2 and 4 weeks after the transplantation of hypoxia-preconditioned dental pulp stem cells (hpDPSCs)

From: Characterization of stable hypoxia-preconditioned dental pulp stem cells compared with mobilized dental pulp stem cells for application for pulp regenerative therapy

Blood tests
Individual number	RBC (10⁶/μL)				WBC (10³/μL)				Platelet count (10³/μL)				Hematocrit (%)
Individual number	pre	7	14	28	pre	7	14	28	pre	7	14	28	pre	7	14	28
control	6.83	6.66	6.28	6.73	10.61	10.41	10.79	15.77	213	200	183	233	45.2	44.2	42.1	44.0
1	6.38	6.54	6.16	5.86	9.13	10.04	9.91	9.37	230	223	260	235	41.9	42.8	40.6	38.8
2	6.62	6.83	6.88	6.20	10.40	9.75	11.26	10.00	296	302	290	264	42.7	44.0	44.2	40.1
3	7.12	6.73	6.94	7.51	8.06	8.49	8.72	8.50	258	252	266	269	46.2	44.2	45.3	48.5
average	6.71	6.70	6.66	6.52	9.20	9.43	9.96	9.29	261.33	259.00	272.00	256.00	43.60	43.67	43.37	42.47
S.D.	0.31	0.12	0.35	0.71	0.96	0.67	1.04	0.61	27.05	32.63	12.96	14.99	1.87	0.62	2.01	4.30
Normal range	5.10-7.47				6.49-16.61				229-401				33.95-47.67
Blood Chemistry examination
Individual number	AST (IU/L)				ALT (IU/L)				Albumin (g/dL)				Globuline (g/dL)				Total choresterol (mg/dL)				Glucose (mg/dL)
Individual number	pre	7	14	28	pre	7	14	28	pre	7	14	28	pre	7	14	28	pre	7	14	28	pre	7	14	28
control	25	27	25	24	41	40	41	35	3.2	3.1	3.1	2.9	2.2	2.1	2.0	2.3	178	182	170	201	94	94	98	93
1	26	23	23	31	55	56	54	91	3.0	2.9	2.9	2.8	2.1	2.5	2.2	2.1	139	157	151	139	94	98	82	88
2	22	20	22	21	45	48	47	44	3.3	3.3	3.4	3.2	2.2	2.3	2.1	2.0	137	150	142	124	94	102	102	98
3	44	27	28	27	42	45	46	48	3.4	3.3	3.3	3.3	2.1	2.1	2.0	2.2	155	149	140	143	87	98	94	95
average	30.7	23.3	24.3	26.3	47.3	49.7	49.0	61.0	3.2	3.2	3.2	3.1	2.1	2.3	2.1	2.1	143.7	152.0	144.3	135.3	91.7	99.3	92.7	93.7
S.D.	9.57	2.87	2.62	4.11	5.56	4.64	3.56	21.28	0.17	0.19	0.22	0.22	0.05	0.16	0.08	0.08	8.06	3.56	4.78	8.18	3.30	1.89	8.22	4.19
Normal range	19-39				20.8-58.8				2.67-3.43				1.8-3.08				101.2-187.6				85.6-110.4
Urinalysis
Individual number	Color			pH			Ketone			Bil			Oc.Bld			Urobil
Individual number	pre	13	27	pre	13	27	pre	13	27	pre	13	27	pre	13	27	pre	13	27
control	0.0	0.0	0.0	6.5	6.0	6.0	0.0	0.0	0.0	1.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0
1	0.0	0.0	0.0	8.5	7.5	7.5	0.0	0.0	0.0	1.0	1.0	0.0	0.0	0.0	4.0	0.0	0.0	0.0
2	0.0	0.0	0.0	7.0	7.0	7.0	0.0	0.0	0.0	1.0	0.0	1.0	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	8.0	8.0	6.5	0.0	0.0	0.0	1.0	0.0	1.0	0.0	0.0	0.0	0.0	0.0	1.0
average	0.0	0.0	0.0	7.8	7.5	7.0	0.0	0.0	0.0	1.0	0.3	0.7	0.0	0.0	1.3	0.0	0.0	0.3
S.D.	0.0	0.0	0.0	0.6	0.4	0.4	0.0	0.0	0.0	0.0	0.5	0.5	0.0	0.0	1.9	0.0	0.0	0.5
Normal range	0.0			5.5-8.5			0-1			0-2			0-3			0-1
Urinalysis
Individual number	U.Vol			S.Grav			Protein(mg/dL)			Glucose (mg/dL)			Na (mEq/L)			K (mEq/L)			Cl (mEq/L)
Individual number	pre	13	27	pre	13	27	pre	13	27	pre	13	27	pre	13	27	pre	13	27	pre	7	14
control	140	240	98	1.047	1.030	1.050	13.4	7.6	24.2	8.0	8.0	9.0	55	37	48	195.6	140.1	248.8	117	54	113
1	60	60	115	1.072	1.046	1.050	48.7	41.3	72.4	10.0	7.0	11.0	49	177	72	366.1	243.1	270.9	260	116	151
2	100	140	135	1.060	1.037	1.050	12.4	17.1	15.2	10.0	14.0	10.0	86	48	76	285.7	185.5	259.6	171	80	126
3	170	180	127	1.048	1.047	1.056	11.0	13.0	34.9	6.0	6.0	12.0	39	42	54	216.9	224.7	280.8	123	108	134
average	110.0	126.7	125.7	1.0600	1.0433	1.0520	24.0	23.8	40.8	8.7	9.0	11.0	58.0	89.0	67.3	289.6	217.8	270.4	184.7	101.3	137.0
S.D.	45.5	49.9	8.2	0.0098	0.0045	0.0028	17.5	12.5	23.7	1.9	3.6	0.8	20.2	62.3	9.6	61.0	24.0	8.7	56.8	15.4	10.4
Normal range	4.4-264			1.0191-1.0891			0-90.51			1.8-16.2			0-165.8			79.1-502.7			24.6-336.2

RBC Erythorocyte count, WBC Lymphocyte count, AST Aspartate transminase, ALT Alanine transminase, Ketone Ketone body, Bil Biliyubin, Oc. Bld Occult blood, Urobil Urobilinogen, U.Vol Urine body, S.Grav Specific gravity

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