Fig. 2

Dynamics of endothelial cell and pericyte populations upon ischemia. a Representative images of endothelial cell (CD31⁺CD146⁻) and pericyte (CD146⁺CD31⁻) populations in gated CD45⁻ populations from ischemic gastrocnemius and soleus muscles. b Representative flow cytometry images of subtypes of pericytes based on Nestin expression. c Quantitation of the endothelial cell population in the CD45⁻ population from ischemic muscles. d Quantitation of the pericyte population in the CD45⁻ population from ischemic muscles. e The ratio of endothelial cell (EC) events/pericyte events. f, g The ratios of type I (f) and type II (g) pericytes in pericyte populations. Student’s t test was used to perform statistical analyses of the data presented in c–g. *p < 0.05; **p < 0.01. The numbers of mice used for flow cytometry on ischemia D0, D2, D4, and D7 were 6, 5, 6, and 6, respectively