Fig. 1

Induction and characterization of iPSCs from ONFH-BMSCs. a Timeline for the reprogramming process. b Cell morphology of ONFH-BMSCs during reprogramming at different times. c Typical image of hES cell-like colony on matrigel (left) and image at high magnification (right). d Alkaline phosphatase-positive colonies. e Immunofluorescence staining of the pluripotency markers. f Teratoma in vivo, which contains all three embryonic germ layers such as respiratory epithelium (upper middle) and gland (lower middle); cartilage (lower left); pigmentary epithelium (upper right); and neural epithelium (lower right). Scale bar indicated 100 μm in (b) and (d), c 200 μm, and e, f 50 μm. D day