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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Lnc13728 facilitates human mesenchymal stem cell adipogenic differentiation via positive regulation of ZBED3 and downregulation of the WNT/β-catenin pathway

Fig. 3

Downregulation of lnc13728 inhibited hADSC adipogenic differentiation. a Lnc13728 was silenced in hADSCs via two independent ASOs (ASO-13728-1 and ASO-13728-2). The knockdown efficiency as compared with the NC was verified by qRT-PCR. b, c qRT-PCR and western blot analyses of the expression of adipogenesis marker genes in hADSCs after knockdown of lnc13728. d Oil red O detection of lipid droplet formation. e Oil red O was extracted from positive-staining cells by isopropanol, and the absorbance was quantified at 570-nm wavelength. The internal control was GAPDH. Data are the means ± SD (n = 3). *p < 0.05; **p < 0.01; ***p < 0.001 compared with the control. Scale bars 200 μm

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Stem Cell Research & Therapy

ISSN: 1757-6512