Fig. 4

Overexpression of lnc13728 promoted hADSC adipogenic differentiation. a Lnc13728 overexpression lentiviral vectors (lenti-13728) and control (lenti-NC) were transfected into hADSCs and indicated by GFP-positive cells. b qRT-PCR verification of the overexpression efficiency of lnc13728. c Oil red O detection of lipid droplet formation. d Oil red O was extracted from positive-staining cells by isopropanol, and the absorbance was quantified at 570-nm wavelength. e, f qRT-PCR and western blot analyses of the expression of adipogenesis marker genes in hADSCs after upregulation of lnc13728. The internal control was GAPDH. Data are the means ± SD (n = 3). *p < 0.05; **p < 0.01; ***p < 0.001 compared with the control. Scale bars 200 μm