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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Lnc13728 facilitates human mesenchymal stem cell adipogenic differentiation via positive regulation of ZBED3 and downregulation of the WNT/β-catenin pathway

Fig. 6

Lnc13728 regulated ZBED3 expression positively, thereby inhibiting the WNT/β-catenin pathway. a Western blot detection of ZBED3 expression after lnc13728 knockdown or overexpression. b Western blot detection of adipogenesis-related proteins after lnc13728 overexpression and ZBED3 knockdown. c hADSCs were treated with Chir99021 (3 μM) after lnc13728 overexpression and expression of WNT-related proteins (β-catenin and p-GSK3β) was detected by western blot. d Western blot detection of β-catenin and p-GSK3β in ZBED3 knockdown hADSCs or control hADSCs. e p-GSK3β and β-catenin were analyzed by western blot in hADSCs undergoing adipogenic differentiation after ZBED3 knockdown and ICG001 (20 μM) treatment. f Immunofluorescence detection of subcellular localization of β-catenin. Scale bar 40 μm. The internal control was GAPDH

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Stem Cell Research & Therapy

ISSN: 1757-6512