Fig. 2From: Apoptosis repressor with caspase recruitment domain (ARC) promotes bone regeneration of bone marrow-derived mesenchymal stem cells by activating Fgf-2/PI3K/Akt signalingIn vitro analysis of the osteogenic differentiation of the ARC-overexpressing BMSCs. a ALP and ARS analysis. ALP staining on day 7 and ARS staining on day 14. b Semiquantitative analysis of ALP activity and semiquantitative analysis of ARS (**p < 0.01). c Immunofluorescence staining detection of OCN protein expression. Nuclei were stained with DAPI. d RT-PCR analysis of osteogenic genes, including ALP, OCN, and Runx2 (*p < 0.05, **p < 0.01)Back to article page