Fig. 2

Ovarian morphology and immunofluorescence analysis of rat ovaries cultured in vitro. a H&E staining of ovaries sections (× 100) and follicles counts. b Immunofluorescence of DAZL in ovaries (red) and statistics of fluorescence intensity. Nuclei were stained by using DAPI (blue, 100×, n ≥ 4). c Immunofluorescence of FOXL2 of ovaries and statistics of fluorescence intensity. Nuclei were stained by using DAPI (blue, × 100, n ≥ 4). Data were represented as mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001