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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: EIF5A2 enhances stemness of epithelial ovarian cancer cells via a E2F1/KLF4 axis

Fig. 5

EIF5A2 modulates the expression of KLF4 through the transcription factor E2F1. a The mRNA levels of 9 candidate genes in SKOV3 cells with EIF5A2 knockdown were detected by qRT-PCR. b Knockdown of EIF5A2 substantially downregulated PLK1 and E2F1 expression in SKOV3 cells detected by western blotting. c Heatmap of EIF5A2, PLK1, and E2F1 mRNA expression of 28 MCAs samples detected by qRT-PCR. d The association between relative EIF5A2 and E2F1 mRNA levels in 28 MCAs samples was analyzed according to the Spearman-correlation factor. e The association between relative EIF5A2 and E2F1 mRNA levels from TCGA database. f Expression of KLF4 in SKOV3 cells transfected with empty vector, EIF5A2 plasmid, si-E2F1, or EIF5A2 plasmid plus si-E2F1. g The WT box indicates that there are 3 potential E2F1 binding sites within 2000 bp upstream of the KLF4 promoter region predicted by JASPAR, and the MUT box indicates the corresponding mutation sequence. The double-luciferase reporter gene experiment confirmed that only Site B transcriptional activity was significantly increased among the 3 binding sites, and the difference in luciferase activity disappeared after mutation. h Expression of pRb, Rb in SKOV3 cells after EIF5A2 downregulation and in HO-8910 cells after EIF5A2 upregulation. i Knockdown of EIF5A2 substantially downregulated Notch1, Hes1, and Hey1 expression in SKOV3 cells detected by western blotting and qRT-PCR

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