Fig. 6

a Protein networks of GATA4 and NKX2-5 in HEK293 cells identified by single BioID experiment reveal overlapping functional protein classes regulating transcriptional pathways. Proteins detected by BioID were filtered using the CRAPome contaminant repository. Proteins seen in more than 10% (41/411) of CRAPome database experiments were discarded. Then, the top 100 interactors with the highest peptide-spectrum match (PSM) values were illustrated for both GATA4 and NKX2-5. b Regulation of the expression of a chamber-specific reporter reveals joint transcriptional modulation by GATA4/NKX2-5 and BET bromodomain inhibitors. Modulation of transcriptional activity resulting from GATA4 binding sites and GATA4 overexpression. NP112 (Nppb promoter sequence) was transfected into COS-1 cells in combination with a GATA4 overexpression vector and luciferase activity was measured in the presence or absence of GATA4-targeted compounds and/or the BET bromodomain inhibitor (+)-JQ1. c Modulation of transcriptional activity resulting from activation of a 3xHA-NKX2-5 luciferase cassette containing NKX2-5 binding sites in combination with GATA4/NKX2-5 overexpression in the presence or absence of GATA4-targeted compounds and/or the BET bromodomain inhibitor (+)-JQ1. NP112—rat minimal BNP promoter-luciferase construct containing GATA4 binding sites, pMT2—plasmid backbone only (no TF overexpression), 3xHA-NKX2-5—promoter-luciferase construct containing three high affinity NKX2-5 binding sites upstream of a rat albumin minimal promoter. Data is presented as mean ± SEM (n ≥ 2, independent experiments)