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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Transplantation of human amniotic epithelial cells promotes morphological and functional regeneration in a rat uterine scar model

Fig. 5

Effects of hAECs on angiogenesis in the injured uteruses. a IHC staining of von Willebrand factor (vWF) reflected the blood vessel density in the uterine scars of different groups at day 30 and 60 after injection of hAECs or PBS (n = 8 uterine horns per group). Scale bars = 25 μm. b IHC staining of VEGFA in the uterine scars at day 30 and 60 after injection of hAECs or PBS in different groups (n = 8 uterine horns per group). Scale bars = 25 μm. c Statistical analysis of the blood vessel density indicated by vWF-positive staining. d The VEGFA expression level was semi-qualified by calculating the percentage of positive cells per field under a magnification of × 400. e Western blot analysis showed that VEGFA expression of the uterine scars in the hAECs group and PBS group at day 5 after injection of hAECs or PBS. f The grayscale values of the western blots were analyzed. The protein level of VEGFA was normalized to that of β-tubulin (n = 5) (*P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; NS, P ≥ 0.05)

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