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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Chitosan hydrogel-loaded MSC-derived extracellular vesicles promote skin rejuvenation by ameliorating the senescence of dermal fibroblasts

Fig. 2

Construction and characterization of the replicative senescence model of dermal fibroblasts. a Schematic diagram of skin dermal fibroblast isolation. b The extracted dermal fibroblasts (DFLs) were verified by Western blotting, using mouse embryonic fibroblasts (NIH3T3) as a positive control and mouse skin keratinocytes (KC) as a negative control. c The morphological comparison of fibroblasts in early generation (P2) and replicative senescence (P8). Scale bar, 200 μm. d Detection of β-galactosidase activity in replicative senescence fibroblasts. Scale bar, 50 μm. e Western blot analysis of the expression of ECM proteins (COL1 and COL3) and matrix metalloproteinases (MMP9 and MMP2). fh Real-time qPCR analysis of aging-related gene expression. Data are presented as the mean ± SD (n = 3; *P < 0.05, **P < 0.01 vs P2)

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