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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Chitosan hydrogel-loaded MSC-derived extracellular vesicles promote skin rejuvenation by ameliorating the senescence of dermal fibroblasts

Fig. 4

CS-EVs promoted the accumulation of ECM in senescent fibroblasts. a Protein levels of Col1 and Col3 were detected by Western blotting in fibroblasts incubated with 75 μg/mL EVs or CS-EVs for 24 h. b Quantification of protein levels normalized to β-actin. c RT-PCR analysis of the expression levels of ECM-associated genes in fibroblasts treated with EVs or CS-EVs for 24 h. d Protein levels of MMPs were detected by Western blotting in fibroblasts incubated with EVs or CS-EVs for 24 h. e Quantification of protein levels normalized to Tubulin and β-actin, respectively. f RT-PCR analysis of the expression levels of MMP and TIMP genes in fibroblasts treated with EVs or CS-EVs for 24 h. Data are presented as the mean ± SD (n = 3; *P < 0.05, **P < 0.01 vs Con; #P < 0.05 vs EVs)

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