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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: NRF2 is required for structural and metabolic maturation of human induced pluripotent stem cell-derived ardiomyocytes

Fig. 2

NRF2 is required for hiPSC-CMs morphology and structure maturation. a Q-PCR and b Western blotting (left) and quantification (right) were used to detect the expression of NRF2 in the siNRF2 and siNC hiPSC-CMs. n = 3. c mRNA expression of ion channels in siNRF2 and siNC hiPSC-CMs. The gene expression is shown normalized first to GAPDH and then to siNC. d α-Actinin (green) and Hoechst (blue) staining of representative siNRF2 and siNC hiPSC-CMs. Scale bar = 10 μm. ei Compared with the siNC hiPSC-CMs, the siNRF2 hiPSC-CMs showed a significant decrease in e cell perimeter, f cell area, g sarcomere length, and i multinucleated cell ratio (n = 3) and h an increase in the circularity index (n > 10 cells per condition, three biological replicates). The means ± SEM are shown. *P < 0.05, **P < 0. 01, ***P < 0. 001, ****P < 0. 0001

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