Fig. 3

Histologic analyses of kidneys of cisplatin-treated mice. C57BL/6 mice received an injection of G-CSF (200 μg/kg per d), G-CSF (200 μg/kg per d)/AMD3100 (5 mg/kg), or saline (as a control of cytokines) into the intraperitoneal space. The day after the last injection of cytokines, single intraperitoneal injections of cisplatin (20 mg/kg body wt) were given to these mice. Morphologic changes in PAS staining are shown. The figures indicated the renal tubules of saline-pretreated mice (a, d), cisplatin + saline-treated mice (b, e), cisplatin + G-CSF-pretreated mice (c, f), and cisplatin + G-CSF/AMD3100-pretreated mice (g, h). Representative data are shown for three independent experiments. Magnification, × 200 in a–c and g and × 400 in d–f and h. Histopathological scoring of cisplatin-induced renal injury (i). Tubular injury was defined as tubular necrosis, cast formation, loss of brush border in the renal tubules, and tubular dilatation. Data are presented as the means ± SD (n = 9 in each group)