Fig. 5

Effects of G-CSF/AMD3100 on renal tubular regeneration. a, b Western blot analyses of PCNA protein. Notably higher PCNA protein expression in the G-CSF/AMD3100 pretreatment group than in the saline or G-CSF-treated group. c–f Immunofluorescent staining tested the expression of Brdu in renal tubular, and red fluorescence represents the nucleus of Brdu⁺ cells. g The number of Brdu⁺ cells/HPF. The figures indicate Brdu⁺ cells in the renal tubules of saline-treated mice (control) (c), cisplatin + saline-treated mice (d), cisplatin + G-CSF-pretreated mice (e), and cisplatin + G-CSF/AMD3100 pretreated mice (f). h–k Immunohistochemical staining tested the expression of Ki-67 in renal tubular. l The number of Ki-67⁺ cells/HPF. The figures indicate Ki-67⁺ cells in the renal tubules of saline-treated mice (control) (h), cisplatin-treated mice (i), cisplatin + G-CSF-pretreated mice (j), and cisplatin + G-CSF/AMD3100-pretreated mice (k). Data represent the mean ± SD (n = 6 per group). *P < 0.05, **P < 0.01, and compared with the cisplatin group; ^#P < 0.05 compared with the control group; ^&P < 0.05 compared with the G-CSF group. Original magnification × 400