Fig. 1
From: Human placenta mesenchymal stem cell-derived exosomes delay H2O2-induced aging in mouse cholangioids
Murine cholangiocyte organoids originating from mature cholangiocytes. a Schematic of cholangioid development. Primary cholangiocytes collected from wild-type C57BL/6 mice by liver duct cell digestion were embedded in Matrigel and covered with expansion medium, allowing them to grow into organoids. b Representative image of long-term cultured cholangioids (P1, passage 1; P2, passage 2; P4, passage 4) (scale bar, 50 μm). c Confocal images of cholangioids expressing specific cholangiocyte markers CK19 (green) and CK7 (red), with blue DAPI staining (scale bar, 20 μm). Left: organoids in two-dimensional form; right: organoids in 3D form. d CK19-positive (green) cholangioids expressing proliferating protein Ki67 (red) (scale bar, 50 μm). e Representative immunohistochemistry images of epithelial cell adhesion molecule (EpCAM) in cholangioids (scale bar, 50 μm)